Secondary structure of RNA phage M12 replicative intermediates in vivo

摘要: Abstract The double strandedness of in vivo pulse-labelled phage M12 Replicative Form (RF) and Intermediate (RI) RNA was determined (1) crude extracts an RNAase I − III Escherichia coli strain (2) subsequent to deproteinization by the phenol procedure. When assayed two different methods, i.e. resistance sensitivity, degree deproteinized material is approximately 50 %. In contrast, lysate, values between 5 27 % are found which increase upon incubation at 37 °C. Evidence presented that -replicating RF RI, as purified deproteinization, derive from primarily single-stranded molecules. However, double-stranded molecules, no longer replicating, accumulate intracellularly during course infection.