Infectious nucleic acids of Escherichia coli bacteriophages. XI. Differentiation between conservatively and semiconservatively replicating RNA intermediates of the bacteriophage M12 by formaldehyde inactivation.

摘要: Abstract Replicative intermediate RNA was purified from cells infected with M12. The infectivity of this replicative inactivated formaldehyde using conditions in which the double-stranded is not affected. Two types could be distinguished by their different sensitivities to formaldehyde. 80% (or more) total at a rate similar that single-stranded RNA. This indicates portion part complete (infectious) viral strand (semiconservative replication mechanism). residual 20% less) more resistant formaldehyde, indicating remains duplex (conservative ratio five one favour semiconservative type found constant throughout cycle. Dimethyl sulphoxide used reduce degree secondary structure In 45% dimethyl sulphoxide, rates for inactivation demonstrated: increased length infectious strand. Treatment 60% converted conservative into completely formaldehyde-resistant form sedimentation properties pure molecules, denatured were shown contain than 0.5% strands. excludes possibility major amounts replicating complementary (minus) strands semiconservatively.