Luteoloside Suppresses Proliferation and Metastasis of Hepatocellular Carcinoma Cells by Inhibition of NLRP3 Inflammasome

摘要: The inflammasome is a multi-protein complex which when activated regulates caspase-1 activation and IL-1β secretion. Inflammasome mediated by NLR proteins that respond to stimuli. Among NLRs, NLRP3 senses the widest array of plays an important role in development many cancer types. However, Whether process hepatocellular carcinoma (HCC) still unknown. Here, anticancer effect luteoloside, naturally occurring flavonoid isolated from medicinal plant Gentiana macrophylla, against HCC cells underlying mechanisms were investigated. Luteoloside significantly inhibited proliferation vitro vivo. Live-cell imaging transwell assays showed migration invasive capacities cells, treated with compared control cells. inhibitory luteoloside on metastasis was also observed vivo male BALB/c-nu/nu mouse lung model. Further studies could reduce intracellular reactive oxygen species (ROS) accumulation. decreased levels ROS induced accompanied decrease expression resulting proteolytic cleavage caspase-1. Inactivation resulted inhibition IL-1β. Thus, exerts its proliferation, invasion through inflammasome. Our results indicate can be potential therapeutic agent not only as adjuvant therapy for HCC, but also, prevention metastatic HCC.