Simultaneous quantification of free fatty acids and acylcarnitines in plasma samples using dansylhydrazine labeling and liquid chromatography-triple quadrupole mass spectrometry.

摘要: Free fatty acid (FFA) and acylcarnitine (AcCar) are key elements of energy metabolism. Dysregulated levels FFA AcCar associated with genetic defects other metabolic disorders. Due to differences in the physicochemical properties these two classes compounds, it is challenging quantify human plasma using a single method. In this work, we developed chemical isotope labeling (CIL)–based liquid chromatography–multiple reaction monitoring (LC-MRM) method simultaneously AcCar. Dansylhydrazine (DnsHz) was used label carboxylic moiety on This resulted formation permanently charged ammonium ion for facile ionization positive mode higher hydrophobicity enhanced retention short-chain analogs reversed-phase LC columns enabled absolute quantification by heavy labeled DnsHz as internal standards. Labeling conditions including concentration freshness cross-linker, time, temperature were optimized. can successfully all short-, medium- long-chain FFAs AcCars greatly sensitivity. Using method, 25 13 be absolutely quantified validated samples within 12 min. Simultaneous CIL-based LC-MRM facilitates investigation metabolism has potential clinical applications.