A practical description of retention in reversed phase chromatography using four parameters

摘要: Retention of small molecules on reversed phase or other hydrophobic stationary phases can be represented by k′ values members a homologous series at varying solvent compositions. The evaluation simple linear relationships leads to the introduction set four parameters that contain all information about retention behaviour this in binary system chosen. It is possible extrapolate hypothetical non-hydrophobic molecule and solvent, polarity which equals phase, characterize properties — eluent system. In addition, chromatographic method for determination ratio column proposed.