Preparative separation of quinolines by centrifugal partition chromatography with gradient elution

摘要: Abstract Centrifugal partition chromatography has been successfully applied to the separation of 2-alkylquinolines from liquid combinatorial synthesis crude samples. Original gradient elution using ternary two-phase solvent systems heptane–water–acetonitrile and heptane–acetonitrile–methanol were used separate them with high purity degrees. Part effluent was monitored evaporative light scattering detection, for direct control, collected fractions analyzed by thin-layer chromatography, GC, nuclear magnetic resonance spectroscopy MS. It thus possible purify in one run more than 3 g mixture only 1.3 l solvents obtain 300 mg several alkylquinolines homologues 99% less 7 h.