Application of stepwise gradients in counter-current chromatography: a rapid and economical strategy for the one-step separation of eight coumarins from Seseli resinosum.

摘要: Abstract The targeted purification of compounds with a broad polarity range from traditional medicinal plants is big challenge for counter-current chromatography (CCC). Gradient elution was introduced in CCC to address this problem. However, once suitable solvent system selected, the separation process requires optimization operational parameters. present study conducted optimize various parameters integrate flow rate and gradients rapid isolation eight coumarins Seseli resinosum single run. An increase temperature 15 °C 35 °C increased stationary phase retention solubility sample, whereas operation time viscosity were decreased. high purity each compound ensured by collecting fractions main peaks while all shoulder mixed separated under same conditions semi-preparative CCC. GC-FID used analyze components phase, which prepared without presaturation save consumption. Finally, purified, including ( 1 ) d -laserpitin, 2 3′S,4′S)-3 ′-angeloyloxy-4′-hydroxy-3′,4′-dihydroseselin, 3 (+)-samidin, 4 ′-acetoxy-4′-angeloyloxy-3′,4′-dihydroseselin, 5 deltoin 6 ), calipteryxin, 7 ′,4′-disenecioyloxy-3′,4′-dihydroseselin, 8 (-)-anomalin. technique has successfully accomplished goal one-step these recovery an economic efficient manner.