Stochastic actin dynamics in lamellipodia reveal parameter space for cell type classification

摘要: The lamellipodium, a thin veil-like structure at the leading edge of motile cells, is fundamental for cell migration and growth. Orchestrated activities membrane components an underlying biopolymer film result in controlled movement whole system. Dynamics two-dimensional motility are primarily driven by actively moving protein lamellipodium. Polymerization actin filaments edge, back-transport network due to myosin motor activity, depolymerization back, diffusive transport monomers front control these dynamics. same molecular prerequisites lamellipodial motion found most eukaryotic cells can function independently body. Here we show that dynamics differ strongly different types according their function. Path finding neuronal growth cones display strong stochastic fluctuations, wound healing fibroblasts locally migrate tissues exhibit reduced fluctuations while fish keratocytes move highly persistently. Nevertheless, experimental analysis computer simulations changes parameters polymerization retrograde alone sufficient utilize same, adaptive machinery this rich variety behaviors.