In vitro growth-inhibitory effect of ethanol GRAS plant and supercritical CO₂ hop extracts on planktonic cultures of oral pathogenic microorganisms.

摘要: Conventional chemical antiseptics used for treatment of oral infections often produce side-effects, which restrict their long-term use. Plants are considered as perspective sources novel natural antiseptics. However, little is still known about inhibitory properties against pathogens. The objective this study was to test in vitro antimicrobial activities generally recognized safe (GRAS) species planktonic cultures cariogenic, periodontal and candidal microorganisms identify active compounds the most extracts. Growth-inhibitory effects ethanol extracts from 109 GRAS plant species, six Humulus lupulus cultivars two hop supercritical CO2 were evaluated using broth microdilution method. analysis done through high-performance liquid chromatography. Best results obtained H. with minimum concentrations (MIC) ≥8 μg/mL ≥16 μg/mL, respectively. CO2H. revealed that α- β-acids main constituents. Capsicum annuum frutescens showed antibacterial effect Streptococcus sobrinus salivarius (MIC=64-128 μg/mL). These strains further inhibited by Zanthoxylum clava-herculis μg/mL) Myristica fragrans (both MIC≥128 latter also exhibited activity Fusobacterium nucleatum (MIC=64 Punica granatum possessed Candida albicans (MIC=128 F. indicate together C. annuum, frutescens, M. fragrans, P. Z. promising materials investigation on new antiseptic agents care products.