Effects of AQ4N and its reduction product on radiation-mediated DNA strand breakage

摘要: Abstract Supercoiled plasmid pBR322 DNA was irradiated in phosphate buffer by 60Co γ-rays at a dose rate 19.26 Gy/min and total of 10 Gy the presence bioreductive antitumour prodrug namely 1,4-bis [{2-(dimethylamino-N-oxide)ethyl} amino] 5, 8-dihydroxyanthracene-9,10-dione (AQ4N) its affinic reduction product 1,4-bis[{2-(dimethylamino)ethyl} 5,8-dihydroxyanthracene-9,10-dione (AQ4) under air nitrogen. AQ4N AQ4 were found to protect against radiation-induced single double strand breakage as assessed agarose gel electrophoresis. The differences between two agents, atmospheres or nitrogen negligible. It also that protection efficiencies compounds pH dependent showed maximum 6. These results indicate radiation damage is an indirect effect since both agents are equally effective despite major their affinity. likely radicals intercepted pH-dependent process.