Determinants of Retroviral-Mediated Gene Delivery to Mouse Spermatogonia

摘要: Spermatogonia represent a new route to transgenesis in mice and potentially some commercially important domesticated animals. In addition, these cells are also potential target for viral integration patients receiving somatic cell gene therapy. But the factors influencing retroviral transduction into spermatogonia not well understood. Because is affected part by proliferative status of host cell, we developed an improved culture system which survive proliferate several days. We used this test ability variety murine avian retroviruses infect spermatogonia. investigated spermatogonia, including infected type envelope, long terminal repeat, method delivery. Here show that many widely vector systems can be successfully transduce at high efficiency. Moreover, delivery MDM2, major downregulator p53, promotes spermatogonial survival culture, suggesting p53 plays role regulating apoptosis induced growth factor deprivation. These results further demonstrate usefulness novel targeting substances interest testis. data have implications improving animal understanding risks associated with