Chronic imaging of cortical sensory map dynamics using a genetically encoded calcium indicator
作者： Matthias Minderer , Wenrui Liu , Lazar T. Sumanovski , Sebastian Kügler , Fritjof Helmchen
DOI: 10.1113/JPHYSIOL.2011.219014
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摘要: 2 Centre of Molecular Physiology the Brain (CMPB) at University Medicine GDepartment Neurology, GGermany Non-technical summary Optical imaging is widely used to map functional areas cerebral cortex. We present a method for fast fluorescence map-level cortical activity using calciumindicatorprotein.Sensory-evokedneuronalactivitycanbeimagedrepeatedlyinthesame mouseover weeks, enablingnew opportunitiesforthe longitudinalstudyof corticalfunctionand dysfunction. hope this will be flexibly applied across different and variety newly developed genetically encoded calcium voltage sensors. Abstract In vivo optical can reveal dynamics large-scale activity, but methodsforchronicrecordingarelimited.Herewepresentatechniqueforlong-terminvestigation wide-field ratiometric indicator (GECI) Yellow Cameleon 3.60. find that GECI signals reportsensory-evokedactivityinanaesthetizedmousesomatosensorycortexwithhighsensitivity spatiotemporal precision, furthermore, measured repeatedly in separate sessions over multiple weeks. This opens new possibilities longitudinal study stability plasticity sensory representations.
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