Solid-phase extraction NMR studies of chromatographic fractions of saponins from Quillaja saponaria.

摘要: The saponin mixture QH-B from the tree Quillaja saponaria var. Molina was fractionated by RP-HPLC in several steps. fractions were analyzed solid-phase extraction NMR (SPE-NMR), a technique combining workup with on-line coupling to an flow probe. Together MALDI-TOF mass spectrometry and comparison chemical shifts of similar saponins, structures both major minor components could be obtained. procedure described is simple method determine structure complex mixture. two found contain at least 28 differing carbohydrate substructures. Eight these have not previously been determined. saponins formed 14 equilibrium pairs migration O-acyl group between adjacent positions on fucosyl residue.