Cadmium inhibits the electron transfer chain and induces reactive oxygen species.
作者： Yudong Wang , Jing Fang , Stephen S Leonard , K.Murali Krishna Rao
DOI: 10.1016/J.FREERADBIOMED.2004.03.010
关键词:
摘要: Recent research indicates that cadmium (Cd) induces oxidative damage in cells; however, the mechanism of stress induced by this metal is unclear. We investigated effects Cd on individual complexes electron transfer chain (ETC) and stimulation reactive oxygen species (ROS) production mitochondria. The activity II (succinate:ubiquinone oxidoreductase) III (ubiquinol:cytochrome c mitochondrial ETC from liver, brain, heart showed greater inhibition than other complexes. stimulated ROS mitochondria all three tissues mentioned above. effect various donors (NADH, succinate, 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinol) was tested separately presence absence Cd. ESR complex might be only site results kinetic studies turnover experiments suggest may bind between semiubiquinone cytochrome b566 Q0 b III, resulting accumulation semiubiquinones at site. semiubiquinones, being unstable, are prone to one molecular form superoxide, providing a possible for Cd-induced generation
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