Facilitating cells enable engraftment of purified fetal liver stem cells in allogeneic recipients.

摘要: It has been reported that while stem cells purified from adult bone marrow engraft in syngeneic recipients, they fail to allogeneic recipients. We have recently shown the addition of as few 30,000 facilitating (CD8+/CD3+/CD45R+/Thy 1.2+/TCR-), a unique marrow-derived population does not possess cell properties, results permanent engraftment major histocompatibility complex (MHC)-disparate host. suggested fetal hematopoietic tissue may be source with enhanced proliferative and self-renewal properties compared marrow. were interested, therefore, whether demonstrated superior capacity In this study, we examined mouse liver Transplantation unmodified into recipients stable multilineage chimerism donor-specific tolerance, indicating pluripotent is present capable Similarly, 2000 3000 sorted (Sca+/c-kit+/Lin-) successfully reconstituted lethally irradiated adults differing only minor antigens. Two thousand 10,000 failed rescue but MHC-matched inoculum resulted sustained production. These results, which parallel our earlier work derived marrow, indicate behaves fashion similar regard transplantation.