Methods for enhancing engraftment of purified hematopoietic stem cells in allogeneic recipients

摘要: The purified hematopoietic stem cells (HSC) transplantation-related complications includes graft failure and rejection. Previous studies have demonstrated that the facilitating (CD8+/TCR-) enhance engraftment of HSC in allogeneic recipients without causing graft-versus-host disease (GVHD). It is hypothesized a specific MHC molecule strongly influences mediated by cells. present invention identifies which loci are essential for curable HSC. Inbred mouse strain combinations congenic all except class I or II were utilized as donors recipients. Recipients reconstituted with 5000 HSX donor mice following 950 cGy total body irradiation. Matching at D not engraftment. If recipient disparate K II-A locus, impaired occurs. appears to be most critical, reflected long-term stable on GVHD. demonstrates maintaining self-renewal capability Moreover, interaction between FC via provides regulatory function promote survival