The clustering of GABA(A) receptor subtypes at inhibitory synapses is facilitated via the direct binding of receptor alpha 2 subunits to gephyrin.

摘要: Classical benzodiazepine sensitive GABA(A) receptor subtypes, the major mediators of fast synaptic inhibition in brain are heteropentamers that can be assembled from alpha1-3/5, beta1-3, and gamma2 subunits, but how neurons orchestrate their selective accumulation at synapses remains obscure. We have identified a 10 amino acid hydrophobic motif within intracellular domain alpha2 subunit regulates receptors inhibitory sites on both axon initial segments dendrites mechanism dependent scaffold protein gephyrin. This was sufficient to target CD4 (cluster differentiation molecule 4) molecules synapses, also critical regulating direct binding subunits gephyrin vitro. Our results thus reveal specific subtypes containing is ability bind