Comparison of in vivo and in vitro toxic effects of microcystin-LR in fasted rats
作者： G.A. Miura , N.A. Robinson , T.W. Geisbert , K.A. Bostian , J.D. White
DOI: 10.1016/0041-0101(89)90031-7
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摘要: The toxic effects of microcystin-LR, a cyclic heptapeptide isolated from the cyanobacterium Microcystis aeruginosa, were studied in fasted rat model and subcellular fractions fasted, toxin-treated control rats. Hepatotoxic lethal dose (100 micrograms/kg) examined 15-90 min post-injection. Elevations serum enzymes, particularly sorbitol dehydrogenase, specific for liver mitochondria, correlated with hepatic damage. Electron micrographs showed progressive cellular disruption, including dilation rough endoplasmic reticulum, incorporation components into cytolysosomes, hydropic mitochondria devoid electron-opaque deposits, loss desmosome-associated intermediate filaments, disruption sinusoidal architecture and, ultimately, lysis hepatocytes. appearance coupled electron transport. Changes plasma membrane-associated cytoskeletal filaments desmosome tonofilaments. In contrast to vivo exposure vitro toxin had no effect on or suggesting that observed indirect may be dependent bioactivation cascade events not supported models.
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sci-hub.se 参考文章(28)
W.H. Adams, H.W. Siegelman, R.D. Stoner, D.N. Slatkin, Toxins of Microcystis aeruginosa and their hematological and histopathological effects ACS Symp. Ser.; (United States). ,vol. 262, ,(1984)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Wayne W. Carmichael, Cindy L.A. Jones, Nik A. Mahmood, Winnie C. Theiss, Palle Krogh, Algal toxins and water‐based diseases Critical Reviews in Environmental Science and Technology. ,vol. 15, pp. 275- 313 ,(1985) , 10.1080/10643388509381734
Winnie C. Theiss, Wayne W. Carmichael, John Wyman, Richard Bruner, Blood pressure and hepatocellular effects of the cyclic heptapeptide toxin produced by the freshwater cyanobacterium (blue-green alga) Microcystis aeruginosa strain PCC-7820. Toxicon. ,vol. 26, pp. 603- 613 ,(1988) , 10.1016/0041-0101(88)90243-7
Edward S. Reynolds, LIVER PARENCHYMAL CELL INJURY : III. The Nature of Calcium-Associated Electron-Opaque Masses in Rat Liver Mitochondria Following Poisoning with Carbon Tetrachloride Journal of Cell Biology. ,vol. 25, pp. 53- 75 ,(1965) , 10.1083/JCB.25.3.53
Maria T.C. Runnegar, Ian R. Falconer, Tom Buckley, Alan R.B. Jackson, Lethal potency and tissue distribution of 125I-labelled toxic peptides from the blue-green alga Microcystis aeruginosa Toxicon. ,vol. 24, pp. 506- 509 ,(1986) , 10.1016/0041-0101(86)90083-8
J P DiMarco, C Hoppel, Hepatic mitochondrial function in ketogenic states. Diabetes, starvation, and after growth hormone administration. Journal of Clinical Investigation. ,vol. 55, pp. 1237- 1244 ,(1975) , 10.1172/JCI108042
Kenneth L. Rinehart, Kenichi. Harada, Michio. Namikoshi, Choryu. Chen, Carl A. Harvis, Murray H. G. Munro, John W. Blunt, Paul E. Mulligan, Val R. Beasley, . et al., Nodularin, microcystin, and the configuration of Adda Journal of the American Chemical Society. ,vol. 110, pp. 8557- 8558 ,(1988) , 10.1021/JA00233A049
Arun S. Dabholkar, Wayne W. Carmichael, Ultrastructural changes in the mouse liver induced by hepatotoxin from the freshwater cyanobacterium microcystis aeruginosa strain 7820 Toxicon. ,vol. 25, pp. 285- 292 ,(1987) , 10.1016/0041-0101(87)90257-1
Kjetil Berg, John Wyman, Wayne Carmichael, Arun Dabholkar, Isolated rat liver perfusion studies with cyclic heptapeptide toxins of Microcystis and Oscillatoria (freshwater cyanobacteria) Toxicon. ,vol. 26, pp. 827- 837 ,(1988) , 10.1016/0041-0101(88)90324-8