Is There Still Room for Novel Viral Pathogens in Pediatric Respiratory Tract Infections?

摘要: Viruses are the most frequent cause of respiratory disease in children. However, despite advanced diagnostic methods currently use, 20 to 50% samples a specific pathogen cannot be detected. In this work, we used metagenomic approach and deep sequencing examine from children with lower upper tract infections that had been previously found negative for 6 bacteria 15 viruses by PCR. Nasal washings 25 (out 250) hospitalized diagnosis pneumonia nasopharyngeal swabs 46 outpatient 526) were studied. DNA reads at least one virus commonly associated was patients, while pathogenic detected remaining 5 For outpatients, all pooled into libraries sequencing. case, 22 sequenced identified, other, but one, both patient groups syncytial virus, coronavirus-OC43, rhinovirus identified. addition, less frequently also found. Saffold not Anellovirus, rotavirus, astrovirus, as well several animal plant groups. No novel Adding up results PCR data, 79.2% 250 76.6% 526 ambulatory patients positive viruses, other children, These suggest type populations studied sampling odds finding novel, clinically relevant pediatric low.