Antigen Expression in Normal and Neoplastic Colonic Mucosa: Three Tissue-specific Antigens Using Monoclonal Antibodies to Isolated Colonic Glands

摘要: Abstract Monoclonal antibodies to normal colonic glands were used localize three tissue-specific antigens in sections of 40 hyperplastic polyps, 29 adenomas, 43 colorectal adenocarcinomas, and blocks or rolls histologically large bowel mucosa. Two identified (designated 3NM 17NM) associated with the mucin vacuole goblet cells third antibody a cell membrane antigen 6NM). Antigen was absent from virtually all carcinoma all, most, polyps. 17NM 21 carcinomas, some antigen-positive present others. 6NM content unchanged compared mucosa 12 decreased somewhat another 20, either markedly depleted 11. increased 22 Three cancer patients synthesized their but cellular distribution abnormal. Antigens most heterogeneity expression seen adenomas. Low concentrations one more significantly frequent ( P