Normal colonic epithelium adheres to carcinoembryonic antigen and type IV collagen
作者： Seiichi Ishii , Glenn Steele , Rosilyn Ford , Giulia Paliotti , Peter Thomas
DOI: 10.1016/0016-5085(94)90015-9
关键词:
摘要: Abstract Background/Aims: Human colorectal carcinoma cells bind to collagen and laminin in the basement membrane as well carcinoembryonic antigen (CEA) on neighboring cells. The purpose of this study was determine whether normal colonie epithelial CEA, collagen, or laminin. Methods: Intact colonic crypts were isolated from mucosa 13 specimens resected for diverticulitis. Colonocytes released by treatment with collagenase deoxyribonuclease tested adhesion type IV a solid-phase assay. Results: Twelve percent 25% colonocytes all bound CEA. seven also but none colonocyte preparations significantly Monoclonal antibodies CEA hyaluronate receptor CD44 enzymatic removal blocked Conclusions: First, use same epitopes adhere Second, through CEA-to-CEA homophilic binding. Third, not laminin, are ligands human colonocytes.
gastrojournal.org
sci-hub.se 参考文章(38)
Song-Yang Yuan, S. Jothy, K. Shirota, Transcription of carcinoembryonic antigen in normal colon and colon carcinoma. In situ hybridization study and implication for a new in vivo functional model. American Journal of Pathology. ,vol. 143, pp. 250- 257 ,(1993)
P. Schlag, K. Koretz, P. Möller, L. Boumsell, Expression of VLA-alpha 2, VLA-alpha 6, and VLA-beta 1 chains in normal mucosa and adenomas of the colon, and in colon carcinomas and their liver metastases. American Journal of Pathology. ,vol. 138, pp. 741- 750 ,(1991)
C Turbide, M Rojas, C P Stanners, N Beauchemin, A mouse carcinoembryonic antigen gene family member is a calcium-dependent cell adhesion molecule. Journal of Biological Chemistry. ,vol. 266, pp. 309- 315 ,(1991) , 10.1016/S0021-9258(18)52436-4
N. Zamcheck, P. Thomas, R. W. Jeanloz, R. A. Byrn, P. Medrek, Effect of Heterogeneity of Carcinoembryonic Antigen on Liver Cell Membrane Binding and Its Kinetics of Removal from Circulation Cancer Research. ,vol. 45, pp. 3137- 3142 ,(1985)
Ursula Hahn, A. Stallmach, E.G. Hahn, E.O. Riecken, Basement membrane components are potent promoters of rat intestinal epithelial cell differentiation in vitro Gastroenterology. ,vol. 98, pp. 322- 335 ,(1990) , 10.1016/0016-5085(90)90821-H
Dennis J. Ahnen, Kinya Kinoshita, Paul K. Nakane, William R. Brown, Differential expression of carcinoembryonic antigen and secretory component during colonic epithelial cell differentiation and in colonic carcinomas Gastroenterology. ,vol. 93, pp. 1330- 1338 ,(1987) , 10.1016/0016-5085(87)90263-0
L. C. Peters, J. S. Brandhorst, M. G. Hanna, Preparation of Immunotherapeutic Autologous Tumor Cell Vaccines from Solid Tumors Cancer Research. ,vol. 39, pp. 1353- 1360 ,(1979)
S Rebstock, K Lucas, J A Thompson, W Zimmermann, cDNA and gene analyses imply a novel structure for a rat carcinoembryonic antigen-related protein. Journal of Biological Chemistry. ,vol. 265, pp. 7872- 7879 ,(1990) , 10.1016/S0021-9258(19)39012-X
Richard V. Benya, Lawrence N. Schmidt, Jasminder Sahi, Thomas J. Layden, Mrinalini C. Rao, Isolation, characterization, and attachment of rabbit distal colon epithelial cells Gastroenterology. ,vol. 101, pp. 692- 702 ,(1991) , 10.1016/0016-5085(91)90527-R
Norman R. Hughes, Ronald S. Walls, John E. Payne, Ronald C. Newland, Antigen Expression in Normal and Neoplastic Colonic Mucosa: Three Tissue-specific Antigens Using Monoclonal Antibodies to Isolated Colonic Glands Cancer Research. ,vol. 46, pp. 2164- 2171 ,(1986)