Molecular-Beacon Multiplex Real-Time PCR Assay for Detection of Vibrio cholerae

作者: Aneta J. Gubala , David F. Proll

DOI: 10.1128/AEM.02597-05

关键词:

摘要: A multiplex real-time PCR assay was developed using molecular beacons for the detection of Vibrio cholerae by targeting four important virulence and regulatory genes. The specificity sensitivity this assay, when tested with pure culture spiked environmental water samples, were high, surpassing those currently published assays organism.

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