Detection of etiological agent for cholera by PCR protocol.

摘要: Background PCR protocol for Vibrio cholerae, the causative agent of diarrheal disease cholera has been described in this report. We report detection species drinking water samples a duplex reaction. The target loci used study were ctxA and tcpA. sensitivity efficiency can be applied epidemic conditions, wherein monitoring organisms is very crucial. Material methods Single step thermocycling programs have reported amplification specific loci. demonstrated that gradient multi-step program more efficient improving by PCR. method preparation template DNA from environmental sample uses filtration followed harvesting possible bacterial residue. This was further treated with proteinase K heat to yield compatible optimized standard strains as well samples. Results simultaneously detect two different cholerae single achieved pathogen 100 cells per specificity primers spiking reaction non-specific template. developed successfully extended Conclusions allows simultaneous genetic enhances provides sensitive tool rapid useful conditions where time factor involved identification