Multiplex real-time PCR detection of Vibrio cholerae

作者: Aneta J. Gubala

DOI: 10.1016/J.MIMET.2005.07.017

关键词:

摘要: … In comparison to the published PCR assays for the detection of V. cholerae, the real-time PCR … This paper describes the development of a fourplex real-time PCR assay that will detect …

参考文章(77)
A Kapley, H J Purohit, Detection of etiological agent for cholera by PCR protocol. Medical Science Monitor. ,vol. 7, pp. 242- 245 ,(2001)
NARAYANAN JOTHIKUMAR, XIAOWEN WANG, MANSEL W. GRIFFITHS, Real-time multiplex SYBR green I-based PCR assay for simultaneous detection of Salmonella serovars and Listeria monocytogenes. Journal of Food Protection. ,vol. 66, pp. 2141- 2145 ,(2003) , 10.4315/0362-028X-66.11.2141
Ghose Ac, Takeda Y, Ramamurthy T, Nair Gb, Nandy Rk, De K, Islam Ms, Modification of the multiplex PCR for unambiguous differentiation of the El Tor & classical biotypes of Vibrio cholerae O1. Indian Journal of Medical Research. ,vol. 114, pp. 77- 82 ,(2001)
M Abbaszadegan, M S Huber, C P Gerba, I L Pepper, Detection of enteroviruses in groundwater with the polymerase chain reaction. Applied and Environmental Microbiology. ,vol. 59, pp. 1318- 1324 ,(1993) , 10.1128/AEM.59.5.1318-1324.1993
R R Colwell, R J Seidler, J Kaper, S W Joseph, S Garges, H Lockman, D Maneval, H Bradford, N Roberts, E Remmers, I Huq, A Huq, Occurrence of Vibrio cholerae serotype O1 in Maryland and Louisiana estuaries. Applied and Environmental Microbiology. ,vol. 41, pp. 555- 558 ,(1981) , 10.1128/AEM.41.2.555-558.1981
M Sandkvist, L O Michel, L P Hough, V M Morales, M Bagdasarian, M Koomey, V J DiRita, M Bagdasarian, General secretion pathway (eps) genes required for toxin secretion and outer membrane biogenesis in Vibrio cholerae. Journal of Bacteriology. ,vol. 179, pp. 6994- 7003 ,(1997) , 10.1128/JB.179.22.6994-7003.1997
Y L Tsai, B H Olson, Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction. Applied and Environmental Microbiology. ,vol. 58, pp. 754- 757 ,(1992) , 10.1128/AEM.58.2.754-757.1992
M S Islam, M K Hasan, M A Miah, M Yunus, K Zaman, M J Albert, Isolation of Vibrio cholerae O139 synonym Bengal from the aquatic environment in Bangladesh: implications for disease transmission. Applied and Environmental Microbiology. ,vol. 60, pp. 1684- 1686 ,(1994) , 10.1128/AEM.60.5.1684-1686.1994