Downregulation of cilia-localized Il-6Rα by 17β-estradiol in mouse and human fallopian tubes

摘要: The action of interleukin-6 (IL-6) impacts female reproduction. Although IL-6 was recently shown to inhibit cilia activity in human fallopian tubes vitro, the molecular mechanisms underlying signaling tubal function remain elusive. Here, we investigate cellular localization, regulation, and possible two receptors (IL-6R alpha gp130) mouse vivo. We show that IL-6R is restricted epithelial cells both tubes. Exogenous 17beta-estradiol (E(2)), but not progesterone (P(4)), causes a time-dependent decrease expression, which blocked by estrogen receptor (ER) antagonist ICI-182,780. Exposure different ER-selective agonists propyl-(1H)-pyrazole-1,3,5-triyl-trisphenol or 2,3-bis-(4-hydroxyphenyl)-propionitrile demonstrated an ER subtype-specific regulation In contrast alpha, gp130 detected mice humans. humans, found muscle decreased periovulatory luteal phases during reproductive cycles, indicating species-specific expression tube. Expression knockout be normal; however, E(2) treatment increased gp130, when compared with wild-type mice. Furthermore, levels parallel estrogenic accelerated oocyte-cumulus complex (OCC) transport Our findings open possibility cilia-specific may play role OCC suggest estrogen-regulatory pathway