Cytotoxicity of Alkyl-lysophospholipid Derivatives and Low-Alkyl-Cleavage Enzyme Activities in Rat Brain Tumor Cells

摘要: Alkyl-lysophospholipids (ALP) and related derivatives inhibited the in vitro incorporation of [3H]thymidine into seven different permanent cell lines derived from rat brain tumors. The cytostatic effect ALP was dependent on dosage incubation time. Naturally occurring 2-lysophosphatidylcholine did not exhibit effects; under these conditions, rates were generally more than 100% controls. trypan blue dye exclusion test, which used to assess severe damage, correlated with extent that by ALP. Preincubation ( rac -1-octadecyl-lyso-glycero-3-phosphocholine) for 8 min a tetrahydropteridine-dependent O -alkyl cleavage enzyme preparation liver microsomes destroyed almost all cytotoxic properties when tested at concentration previously tumor growth 50%. [3H]Thymidine greater astrocytoma cells incubated after exposure alkyl enzyme. Comparison microsomal activities alkyl-cleavage present 78-FR-G-299 pleomorphic glioma 78-FR-G-219/S4 found normal skin fibroblasts livers revealed markedly reduced activity neoplastic lines. Moreover, those resistant cytotoxicity (pleomorphic glioma, 78-FR-G-219/S4) had 3-fold higher sensitive line (astrocytoma cells, 78-FR-G-299). Our results indicate low-alkyl-cleavage comparison might be factor explaining relatively high cells.