ERK5 regulates invasiveness of osteosarcoma by inducing MMP-9
作者： Sang-Min Kim , Hyewon Lee , Youn-Soo Park , Youbin Lee , Sung Wook Seo
DOI: 10.1002/JOR.22025
关键词:
摘要: The purpose of this study is to determine the role ERK5 in cellular invasion osteosarcoma (OS). human OS cell line (MG63, SaOS, and U2OS) primary cells were used for study. expression MMP-9 each was examined by western blot or RT-PCR. To evaluate biological ERK5, proliferation assay (MTT) (BD Matrigel™) performed after silencing using siRNA. MMPs expressions analyzed RT-PCR zymography ERK5. distinctly overexpressed U2OS cell. Both them also expressed MMP-9, which not shown MG63 SaOS did suppress cells. However, significantly reduced number invading assay. specifically showed that enzyme activity suppression. regulates inducing expression. Therefore, may be a new therapeutic target invasive expressing MMP-9.
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