Ovarian cancer-derived exosomes promote tumour metastasis in vivo: an effect modulated by the invasiveness capacity of their originating cells.

摘要: Exosomes are small nanovesicles that carry bioactive molecules which can be delivered to neighbouring cells modify their biological functions. Studies have showed exosomes from ovarian cancer (OVCA) alter the cell migration and proliferation of within tumour microenvironment, an effect modulated by invasiveness capacity originating cells. Using OVCA line xenograph mouse model, we derived a high (exo-SKOV-3) promoted metastasis in vivo compared with low (exo-OVCAR-3). Analysis anin imaging system (IVIS) revealed exo-SKOV-3 formed metastatic niches, whereas exo-OVCAR-3 colonies clustered close site injection. Interestingly, kinetic parameters half-maximal stimulatory time (ST50) growth (4.0 ± 0.31 weeks) was significantly lower ST50 mice injected (4.5 0.32 weeks). However, number metastic nodes higher exo-OVCAR-3. quantitative mass spectrometry approach (SWATH MS/MS) followed bioinformatics analysis using Ingenuity Pathway (IPA), identified total 771 proteins. Furthermore, 40 these proteins were differentially expressed tissues exo-OVCAR-3, associated Wnt canonical pathway (β-catenin). Finally, set had elevated expression circulating association metastasis. These observations suggest exosomal signalling plays important role