Impact of C24:0 on actin-microtubule interaction in human neuronal SK-N-BE cells: evaluation by FRET confocal spectral imaging microscopy after dual staining with rhodamine-phalloidin and tubulin tracker green.

摘要: Disorganization of the cytoskeleton neurons has major consequences on transport neurotransmitters via microtubule network. The interaction proteins (actin and tubulin) was studied in neuronal SK-N-BE cells treated with tetracosanoic acid (C24:0), which is cytotoxic increased Alzheimer's disease patients. When were C24:0, mitochondrial dysfunctions a non-apoptotic mode cell death observed. Fluorescence microscopy revealed shrunken perinuclear condensation actin tubulin. Impact C24:0 actin-microtubule human cells: evaluation by FRET confocal spectral imaging after dual staining rhodamine-phalloidin tubulin tracker green After an antibody raised against α-/β-tubulin, modifications F-actin α-/β-tubulin levels detected flow cytometry. Lower α-tubulin found Western blotting. In C24:0-treated cells, analysis fluorescence recovery photobleaching (FRAP) measured proved existence resonance energy transfer (FRET) when stained demonstrating co-localization/interaction. control no Our data demonstrate quantitative changes tubulin, modified interactions between C24:0. They also show that interesting method for specifying impact compounds proteins.