We heart cultured hearts. A comparative review of methodologies for targeted differentiation and maintenance of cardiomyocytes derived from pluripotent and multipotent stem cells

摘要: Human cell lines, including disease lines are often superior to routine animal models for the purposes of rapid and safe assessment effects different agents that may modulate myocardial functioning under physiological pathological conditions. There several currently existing methodologies derivation cardiomyocyte-like cells by targeted differentiation from pluripotent reprogramming/ transdifferentiation other types (multipotent progenitors, somatic cells, etc). The present paper reviews potential sources capable along cardiomyocyte lineage; differentiation, outlining specificities each method; markers mesodermal cardiogenic lineage. yield robustly beating expressing cardio-specific proteins derived any methods, however, still rarely exceeds 70-90 %, even with newly developed approaches increasing capacity. is significant variance in results obtained research groups between experiments carried out same laboratory, type general protocol. Derivation new human multipotent stem according standardised protocols completely defined; xeno-free conditions increase reliability reproducibility speed up development clinical applications.