Development of cardiomyocytes from human ES cells.

摘要: Publisher Summary Cell transplantation is emerging as a novel strategy for myocardial regeneration, but it has been hampered by the lack of source human cardiomyocytes. Similarly, cardiomyocyte cell line significantly limited variety experimental procedures. The recent advances in embryonic stem cells (ES) research suggests possible solution this sourcing problem because these unique can be propagated mass vitro and coaxed to differentiate into desired lineage. One most fascinating important aspects ES lines their ability advanced derivatives all three germ layers. A prerequisite differentiation cultivation three-dimensional aggregates. These aggregates, termed embryoid bodies (EBs) contain differentiating tissue from Using EB system cardiomyocytes reproducibly generated cells. This chapter describes methodologies associated with cardiomyocytes, well techniques used studying structural functional properties