Regulation of proliferation of the fetal myocardium.

摘要: Growth of the myocardium involves completion a fixed number rounds cell division during embryonic and fetal stages followed by entry into postmitotic state hypertrophy cardiomyocytes later heart growth. It has been suggested that at time its determination in early embryo, is programmed for limited divisions, after which transition to occurs autonomously. The proliferative response cultured chick was explored four culture settings: monolayer culture, collagen lattice organ reaggregated cardiomyocyte tissue (cardiomyocyte spheroid culture), pieces ventricle wall. Several growth factors were identified their ability stimulate DNA synthesis cardiomyocytes, incorporation 5-bromodeoxyuridine (BrdU). serine proteases thrombin trypsin, fibroblast factor-2 (FGF-2), transforming factor-α (TGF-α), insulin-like factor-II (IGF-II) all show factor activity but only three-dimensional myocardial tissue, not maintained culture. Thus, proliferation phase, controlled solely internal, cell-autonomous programs, subject external regulation family peptide factors. unconventional setting required demonstrate responsiveness challenge. © 2000 Wiley-Liss, Inc.