Analysis of population cytokinetics of chick myocardial cells in tissue culture

摘要: Abstract The growth of embryonic chick cardiac myocytes and fibroblasts in tissue culture was evaluated by the kinetics nuclear labeling during continuous exosure to [ 3 H]thymidine. fraction mitotically active cells, mean intermitotic period, population doubling times were determined each cell type weeks culture. After 24 hr culture, 90% muscle cells with minimal 65 hr. By 17 days only 5% continued divide greater than 3000 Primarily, lengthening due a withdrawal from mitotic cycle much less period. Growth hearts 4 10 development also compared. Muscle younger displayed activity those older at equivalent