Rat myocardial cells in vitro: mitosis and differentiated properties.

摘要: Ventricular heart cultures were prepared from newborn rats and examined in Rose chambers by high resolution, phase-contrast optics. Details of the procedure are given for obtaining a yield myocardial or endothelial cells. Photographic records obtained living cells using photomicrography, cinematography, speed filming to document cytological differences between cells, details mitosis two cell types, patterns contractility. Myocardial can be distinguished state following criteria; dense cytoplasm; giant pleomorphic mitochondria sarcosomes: presence small, round nuclei; binucleation; one round, nucleoli; specialized Golgi apparatus around nucleus; myofobrils; intercalated discs; myopodia; size; failure migration; spontaneous contractions; formation synchronized networks; flattened appearance with adhesion other during mitosis. Contracting shown undergo Contractions become weaker at metaphase then cease anaphase. Daughter may resume contractions. Organized myofibrils generally not detected mitotic periods when contractions minimal absent. It is proposed that transient disorganization mid-mitosis reorganized daughter cell(s). suggested there competition energy time spindle changes chromosome movements, so priority process rather than myofibrillar Myofibrillogenesis considered relatively rapid process. The average 2.5 hr fore 6.1 Failure cytokinesis frequently occurs dividing results binucleated A sequence, presented which undergoes an abormal multipolar mitosis, leading polynucleation. incorporate tritiated thymidine into nuclear DNA. question differentiation reviewed. concluded no longer cited support dogma, differentiated do divide. However, these temporary phenomena metaphase-anaphase stages.