Quantitative Detection of Mycoplasma DNA Using Competitive PCR

摘要: Mycoplasma are free-living pleomorphic prokaryotes that can pass through regular bacteriological filters. These organisms have a small genome with low G + C content, and lack the rigid peptidoglycan cell wall of eubacteria. There more than 80 species known to date, major culture contaminants human, bovine, or porcine origin being: orale, M. fermentants, hyorhinus, arginini, salivarium, Acholeplasma laidlawii (McGarrity et al., 1985). More 15–30% all lines used contaminated mycoplasma (Mowles 1988; Gignac 1991). grow in supernatant attached extracellular surface membranes. The growth these microorganisms may not necessarily destroy cells but produces significant changes medium composition, which leads alterations host metabolism, thus rendering infected unacceptable for use any research diagnostic procedure. Cell infections by various species, as well their effects, documented. Some effects on cultures include induction suppression enzymes cytokines, alteration antigenicity, interference viral expression, chromosomal breaks. A few also been reported produce transformation (Macpherson Russel, 1966).