Quantitative competitive polymerase chain reaction for accurate quantitation of HIV DNA and RNA species

摘要: Inherent features of the PCR make this procedure suboptimal for quantitative applications. For typical clinical specimens, absolute amount product derived from does not always bear a consistent relationship to target sequence present at start reaction. Competitive approaches quantitation nucleic acid sequences overcome limitations basic methods quantitation. We have developed competitive method known as HIV DNA and RNA sequences. Key technique include quantitation, based on relative amounts products produced template introduced into test stringent internal control all reactions, including reverse transcription step in PCR. The is suitable analysis specimens may be particularly valuable accurate viral load patients undergoing treatment with experimental therapies.