Primary structure of an α-tubulin gene of Physarum polycephalum

摘要: Abstract An α-tubulin gene of Physarum was isolated as a phage-λ NM1149 recombinant (designated NαTu). Phage-λ NαTu contained 4700 base-pair Hind III nuclear DNA fragment an allele the alt B locus (one four unlinked loci). Subfragments insert were cloned into phage M13 and nucleotide sequence determined by dideoxy chain termination method. The start point transcription identified primer extension putative polyadenylation site located S 1 nuclease analysis. 4650 spans complete gene; sequences upstream from 5′ end contain RNA promoter elements (the TATA CCAAT boxes). encoding contains seven intervening sequences, ranging 63 to 222 nucleotides in size. exons have that is identical with cDNA clone, except for three base changes, one leading Val codon place Met codon, another Glu Asp third change silent. genomic clone provides coding last 26 amino acid residues missing clone. new data indicate has C-terminal methionine not tyrosine which been found all genes sequenced date.