A new bile acid conjugate, ciliatocholic acid, from bovine gall bladder bile.

摘要: This study was carried out to investigate the occurrence of ciliatocholic acid in bovine gall bladder bile. Ciliatocholic synthesized according method described by Bergstrom and Norman for synthesis taurocholic acid. Elemental analysis, melting point, infrared spectrum this substance were determined. An isolation procedure established stepwise elution with an HCl-ethanol solvent system using a Dowex-1 anion exchange resin column chromatographic technique. amounting 158 mug (as ciliatine) per 100 ml bile found fraction eluted 0.01 N HCl 50% ethanol. coumpound purified preparative thin-layer chromatography confirmed be from hydrolytic stability, phosphorus determination, behavior. Thus, contains small amount