Intracellular glutathione pools are heterogeneously concentrated.
作者： Davide Montero , Christine Tachibana , Jakob Rahr Winther , Christian Appenzeller-Herzog
DOI: 10.1016/J.REDOX.2013.10.005
关键词:
摘要: Glutathione is present in millimolar concentrations in the cell, but its relative distribution among cellular compartments remains elusive. We have chosen the endoplasmic reticulum (ER) as an example organelle to study compartment-specific glutathione levels. Using a glutaredoxin sensor (sCGrx1p ER), which rapidly and specifically equilibrates with the reduced glutathione (GSH)–glutathione disulfide (GSSG) redox couple with known equilibrium constant, we showed that the [GSH]:[GSSG] ratio in the ER of intact HeLa cells is …
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