Yeast functional analysis: identification of two essential genes involved in ER to Golgi trafficking.
作者： Naïma Belgareh-Touzé , Marisol Corral-Debrinski , Heike Launhardt , Jean-Marc Galan , Thomas Munder
DOI: 10.1034/J.1600-0854.2003.00116.X
关键词:
摘要: We screened for genes potentially involved in the secretory and vacuolar pathways a collection of 61 yeast strains, each bearing an essential orphan gene regulated by tetO7-CYC1 promoter that can be down-regulated doxycycline. After down-regulating expression these genes, we performed systematic Western blot analysis markers undergo post-translational modifications their intracellular trafficking. Accumulation protein precursors, revealed immunoblot analysis, indicates defects pathway or associated biochemical modifications. screening whole collection, identified two ER to Golgi trafficking: RER2, cis-prenyl transferase, USE1, function which was unknown. demonstrated repression USE1 also leads BiP secretion, therefore likely affects retrograde, addition anterograde, The includes trafficking were conveniently repressed without resulting growth defects.
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