Large-scale analysis of the yeast genome by transposon tagging and gene disruption

摘要: Economical methods by which gene function may be analysed on a genomic scale are relatively scarce. To fill this need, we have developed transposon-tagging strategy for the genome-wide analysis of disruption phenotypes, expression and protein localization, applied method to large-scale in budding yeast Saccharomyces cerevisiae. Here present largest collection defined mutants ever generated within single genetic background--a over 11,000 strains, each carrying transposon inserted region genome expressed during vegetative growth and/or sporulation. These insertions affect nearly 2,000 annotated genes, representing about one-third 6,200 predicted genes genome. We used determine phenotypes 8,000 strains using 20 different conditions; resulting data sets were clustered identify groups functionally related genes. also identified 300 previously non-annotated open reading frames indirect immunofluorescence 1,300 transposon-tagged proteins. In total, our study encompasses 260,000 points, constituting functional undertaken.