Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
作者： Henry A. Erlich , Kary B. Mullis , Glenn Horn , Randall K. Saiki
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摘要: Single or multiple nucleotide variations in nucleic acid sequence can be detected acids by a process whereby the sample suspected of containing relevant is repeatedly treated with primers, triphosphates, and an agent for polymerization triphosphates then denatured, which amplifies variation if it present. In one embodiment, spotted on membrane labeled sequence-specific oligonucleotide probe. Hybridization probe to label
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