Levels of Cu-Zn and Mn superoxide dismutases in rat liver during development

摘要: Superoxide dismutase is the main scavenger of superoxide radicals in mammalian body. The liver has high levels two types enzymes, cytosolic Cu-Zn and mitochondrial Mn enzymes. aim present study was to investigate content distinct dismutases during perinatal transition from a hypoxic hyperoxic environment. Both isozymes were purified homogeneity used as immunogens rabbits. Antisera raised found recognize only polypeptides molecular weight 16,900 or 23,400, which correspond dismutases, respectively. It that level enzymatic activity protein assessed by immunoquantitation increased 10-fold postnatal period, reaching adult 3 weeks. In contrast, amount twofold first week life. Neither showed an alteration specific apparent rat livers ontogeny. These results show intracellular are differentially elevated period. suggested each plays different physiological role for scavenging function hypoxic/hyperoxic environment at birth.