Rapid detection of bacteria based on homogenous immunoassay using chitosan modified quantum dots
作者： Üzeyir Dogan , Esin Kasap , Demet Cetin , Zekiye Suludere , Ismail Hakkı Boyaci
DOI: 10.1016/J.SNB.2016.04.081
关键词:
摘要: Abstract In this study, a fast and sensitive sandwich assay has been developed with the combination of immunomagnetic separation (IMS) fluorescence techniques to enumerate Escherichia coli ( E.coli ). Iron oxide core gold shell (Fe 3 O 4 @Au) magnetic nanoparticles were prepared modified biotinylated antibodies specific . Fluorescence labels have constructed by preparing chitosan coated CdTe quantum dots (CdTe QDs) diameter 3 ± 1 nm. The amounts QDs are optimized get best sensitivity. calibration graph intensity versus concentration was linear in range 10 2 –10 8 cfu mL −1 coefficient determination (R ) 0.9905. limit detection (LOD) quantification (LOQ) values calculated as 30 100 cfu mL , respectively. Selectivity method is examined applying same procedure bacteria, namely Enterobacter aerogenes E. ), dissolvens Staphylococcus aureus S. Pseudomonas aeruginosa P. no interference observed. applicability also spiked urine samples. As result, it found that, novel target rapid total analysis time less than 120 min.
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