作者: R. Zakut-Houri , B. Bienz-Tadmor , D. Givol , M. Oren
DOI: 10.1002/J.1460-2075.1985.TB03768.X
关键词:
摘要: A 2.5-kb cDNA clone for human p53 tumor antigen has been isolated. This contains the entire coding region including 135 bp upstream of first ATG. Comparison nucleotide sequence and mouse demonstrates that ATG in corresponds to second (codon No. 4) p53. The comprises 393 residues is longer than due six additional codons present at corresponding exon 4 gene. DNA homology between regions 81% conservation not equally distributed along molecule. When inserted into SV40-based expression vectors successfully directs production a polypeptide with an apparent mol. wt. 55 kd which can be precipitated by monoclonal antibodies