Differential Response of Extracellular Proteases of Trichoderma Harzianum Against Fungal Phytopathogens.

摘要: In the present study, production of extracellular proteases by Trichoderma harzianum was evaluated based on relative gene expression and spectrophotometric assay. The fungal isolates were grown in Czapek Dox Broth medium supplemented with deactivated mycelium plant pathogens such as Fusarium oxysporum, Colletotrichum capsici, Gloeocercospora sorghi, truncatum. maximum protease activity detected after 48 h incubation against spp. Similarly qRT-PCR, four varied from to 96 host a time-independent manner. Among proteases, statistically significant upregulation asp, srp observed spp., followed F. oxysporum. But case pepM22, variation enzyme assay qRT-PCR at different time intervals various phytopathogens could be due limitation using casein substrate for all types or protease-encoding transcripts selected which may not true representative total activity.