Fermentative hydrogen and methane production from microalgal biomass (Chlorella vulgaris) in a two-stage combined process

摘要: This study investigated the hydrogen fermentation in a two-stage combined process (combination of dark for (H2) production from microalgae and methane (CH4) residues following H2 process). Microalgae Chlorella vulgaris culture was used as substrate. The C. ranges 1.75 ± 1.50–19 ± 2.94 ml g-VS−1 at different substrate dosages without enzymatic pre-treatment. A seven-fold increase yields (19 ± 2.94–135 ± 3.11 ml g-VS−1) observed with pre-treatment (Onozuka R-10 Macerozyme R-10) microalgae. results CH4 show that could be increased 245 ± 2.46 to 414 ± 2.45 ml by using pretreatment. In addition, yield (14.86 kJ/g-VS) has approximately same order magnitude comparison (14.46 kJ/g-VS) terms energy production.