A peripheral blood diagnostic test for acute rejection in renal transplantation.

摘要: Monitoring of renal graft status through peripheral blood (PB) rather than invasive biopsy is important as it will lessen the risk infection and other stresses, while reducing costs rejection diagnosis. Blood gene biomarker panels were discovered by microarrays at a single center subsequently validated cross-validated QPCR in NIH SNSO1 randomized study from 12 US pediatric transplant programs. A total 367 unique human PB samples, each paired with for centralized, blinded phenotype classification, analyzed (115 acute (AR), 180 stable 72 causes injury). Of differentially expressed genes microarray, Q-PCR analysis five gene-set (DUSP1, PBEF1, PSEN1, MAPK9 NKTR) classified AR high accuracy. logistic regression model was built on independent training-set (n = 47) test-set 198)samples, discriminating STA 91% sensitivity 94% specificity all non-AR phenotypes 90% specificity. The 5-gene set can diagnose potentially avoiding need biopsy. These data support conduct prospective to validate clinical predictive utility this diagnostic tool.