Molecular evidence of injury and inflammation in normal and fibrotic renal allografts one year posttransplant.

摘要: Introduction. Factors contributing to kidney transplant fibrosis remain incompletely understood, particularly in the absence of acute complications. Methods. Baseline and 1-year surveillance biopsies from 15 uncomplicated living donor transplants were subjected microarray quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses examine changes gene expression patterns over time. Biopsy pairs purposefully selected allografts with no history complications divided into those that histologically normal (n=7) had developed subclinical interstitial (n=8) at 1 year. Results. Compared paired baseline specimens, levels 3578 probesets found altered all studied. A large proportion up-regulated genes this transplant-associated profile functionally linked inflammation, immunity, or response injury. These included components inflammation-related signaling pathways (integrin, interferon, Toll-like receptor) as well individual mediators inflammatory immune responses. An additional 2884 demonstrated fibrotic grafts only The products fibrosis-associated also predominantly inflammation function, suggesting exaggerated activity within grafts. qRT-PCR confirmed predicted for transcripts profiles. Conclusions. Transcriptional profiles renal indicate there is ongoing injury year compared immediate posttransplant period. Subclinical development during first associated up-regulation genes.