Enhanced antitumor efficacy of folate modified amphiphilic nanoparticles through co-delivery of chemotherapeutic drugs and genes.
作者： Bojie Yu , Cui Tang , Chunhua Yin
DOI: 10.1016/J.BIOMATERIALS.2014.04.095
关键词:
摘要: Abstract Folate (FA) modified amphiphilic linoleic acid (LA) and poly (β-malic acid) (PMLA) double grafted chitosan (LMC) nanoparticles (NPs) with optimum grafting degrees of hydrophobic LA hydrophilic PMLA were developed for the co-delivery paclitaxel (PTX) survivin shRNA-expressing plasmid (iSur-pDNA). The resultant NPs exhibited particle size 161 nm zeta potential 43 mV. FA modification increasing correlated suppressed protein adsorption, inhibited release PTX, accelerated dissociation pDNA. PTX loading, cellular uptake, nuclear accumulation pDNA, in vitro gene silencing efficiency, cell growth inhibition promoted by higher degree LA, but impeded PMLA. In tumor-bearing mice, iSur-pDNA enhanced antitumor efficacy prolonged survival period as compared single delivery or iSur-pDNA. These results indicated that LMC could serve a promising platform drugs genes, highlighted importance adjusting degrees.
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